Low temperature spray dryer-Experimental

Low temperature spray dryer - Experimental  machineBrief description

1， Experimental low temperature spray dryer；

2， Processing volume 50-1000ml per hour；

3， Incoming wind temperature: 30-140 degrees；

4， It can be upgraded to a high and low temperature spray dryer；

Low temperature spray dryer - Experimental  machineExperimental Report

1，Sample introduction

Raw state of material

2，Precipitation state after centrifugation：

Condition: 0.1 g， above mentioned powder added SOD extract， 8000g / 10 mins; PDC reagent added and centrifuged, the situation is similar.

Conclusion

1, #1 and #8: The pellets in the upper layer of centrifugal precipitation are fine, but there are a few coarse particles in the bottom layer; The overall color of #1 is deeper than #8. Supernatant OD600 after centrifugation [~0.2-0.4].

2, #2: The particles are fine and there are no coarse particles. Supernatant OD600 after centrifugation [~0.2-0.4].

3, #7:The whole sample is rough, the texture is uneven, and the color is yellowing; Supernatant OD600 after centrifugation [~1.6-1.8].

3, Protein content of product 4, PDC enzyme activity measurement

5, SOD enzyme activity measurement

Low temperature spray dryer - Experimental  machine Conclusion

1, From the point of view of physical treatment (uniformity), the treatment effect of low temperature spray drying is the best, and the treatment effect of buying capsule inclusions is the worst. The largest number of colored extracts were purchased from capsule inclusions, and the components and functions of the extracts could not be determined.

2, Compared with dry weight, the PDC enzyme activity of freeze-dried material and low temperature spray drying treatment was the highest. The enzyme activity of high temperature spray drying is about 20% lower than that of low temperature spray drying. The enzyme activity of the capsule was the lowest, which decreased about 85% compared with the low temperature spray drying treatment. Compared with unit protease activity, although the activity of PDC was the highest in low temperature spray drying and freeze-dried treatments, in fact, the four groups of data were similar, reflecting the stability of PDC as the key enzyme for metabolism.

3, Compared with freeze-drying method, the enzyme activity of SOD in low temperature spray drying was the highest, which was about 25% higher than that of freeze-drying. The enzyme activity of the material in the capsule is close to that of the freeze-drying method. The lowest enzyme activity is high temperature spray drying, only about 50% of low temperature spray drying. Compared with the unit protease activity, the material in the capsule has obvious SOD activity, which is the most characteristic, but the activity is easily destroyed in ultrasonic treatment.

Brief conclusion:

1) the uniformity of sample treated by low temperature spray drying is optimal.

2) drying method has little effect on protein content.

3) the lyophilization treatment and the low temperature spray drying treatment had higher enzyme activity.

4) the content of protein in the capsule is the least, and it has high antioxidant activity and very low cellular activity.

5) the way the cells were broken by ultrasound was not conducive to the maintenance of cell activity and antioxidant activity.

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