VECTOR B-1155*Jacalin Biotinylated Jacalin

Jacalin Biotinylated Jacalin
Detection of Glycoproteins using Lectins in Histochemistry,
ELISA, and Western Blot Applications

isolated from Artocarpus integrifolia （Jackfruit） seeds 
Jacalin is a lectin composed of four subunits, two of approximay 10,000 daltons and two of 16,000 daltons each. This 50,000 dalton glycoprotein appears to bind only O-glycosidically linked oligosaccharides, preferring the structure galactosyl （b-1,3） N-acetylgalactosamine. This structure （the so-called “T-antigen”） is the oligosaccharide to which peanut agglutinin binds. However, unlike PNA, Jacalin will bind this structure even in a mono- or disialylated form. This lectin has been used to purify human IgA, since no other human immunoglobulin class binds Jacalin （references available upon request）. The specificity of this lectin also affords the opportunity to localize or isolate glycoproteins with O-glycosidically linked oligosaccharide side chains.

This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by the VECTASTAIN® ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation. 
Inhibiting/Eluting Sugar: 800 mM galactose or 100 mM melibiose